DETERMINATION OF FREE AND TOTAL CHOLESTEROL BY DIRECT CHLOROFORM EXTRACTION
نویسندگان
چکیده
منابع مشابه
Determination of free and total cholesterol by direct chloroform extraction.
Since the Liebermann-Burchard reaction was adopted by Grigaut (1) for the quantitative determination of serum total cholesterol, many modified procedures of the original method have been made. The various procedures have differed in extraction method and in the reagents used in the color development. The separation of cholesterol from its esters with digitonin has had limited application becaus...
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15 صفحه اولMethod for fully automated determination of total cholesterol in blood serum, including saponification and extraction.
A fully automated method has been developed for the determination of total cholesterol in blood serum, using the AutoAnalyzer system. According to the new method, based on the cholesterol determination of Abell et a!. (1), the serum sample is saponified and subsequently extracted with carbon tetrachloride. After treatment with Liebermann-Burchard reagent, the amount of cholesterol is determined...
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An enzymatic method is described for determination of total serum cholesterol by use of a single aqueous reagent. The method requires no prior treatment of sample and the Calibration curve is linear to 600 mg/dl. Cholesterol esters are hydrolyzed to free cholesterol by cholesterol ester hydrolase (EC 3.1.1.13). The free cholesterol produced is oxidized by cholesterol oxidase to cholest-4-en-3-o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1949
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)56747-8